200 Kd Standard File Name C:\Documents and Settings\Ayres Research Group\My Documents\Leeanne\200 Kd Standard.afe6 Collection Version 6.0.1.10 Collection Operator AYRESGPC\Ayres Research Group (AYRESGPC\Ayres Research Group (Ayres Research Group)) Processing Operator AYRESGPC\Ayres Research Group (Ayres Research Group) dn/dc 0.000000000e+000 A2 0.000000000e+000 UV Ext. Coef. 0.000000000e+000 Mark-Houwink-Sakurada K 0.000000000e+000 Mark-Houwink-Sakurada a 0 Reference Wavelength 0.000000000e+000 Reference Temperature 2.500000000e+001 Concentration 0.000000000e+000 Collection procedure template Despiking Procedure template Define Baselines template Alignment template Broaden template Define Peaks template Molar Mass & Radius from LS template Results fitting : Molar Mass Results fitting : rms radius Results fitting : mean square radius Distribution Analysis template mean square radius vs volume LS + RI online Abscissa Units min Concentration Source RI Flow Rate 1.000000000e+000 thf System solvent Reference Refractive Index 1.402938872e+000 Temperature Correction Enabled no Reference Temperature 2.500000000e+001 Reference Wavelength 6.328000000e+002 Refractive Index 1.402273917e+000 Rayleigh Ratio 3.690183168e-006 Viscosity 4.560000000e-001 Refractive Index Model Model Name Polynomial Equation N(λ) = 1.40282 + 0.0038323 λ² - 0 λ^4 - 0- 0.000378058 * 25 Rayleigh Ratio Model Model Name Corrected Lambda^4 Equation R(λ, T) = 4.28e-006 * (632.8/λ)^4 * (N(λ)/N(632.8))²[(N(λ) - 1)/(N(632.8) - 1)]² Viscosity Model Model Name Linear Equation η(T) = 0.00456 + -4.9e-005 * (T - 25) Thermal Expansion Model Model Name Fixed Equation fixed at 0 @ 0 °C injector Injected Volume 1.000000000e+002 Auto Inject Signal no TREOS Temperature Control no Band Broadening Correction n/a Disable Data Collection no Physical Instrument Name miniDAWN TREOS Physical Instrument Location WYATT-333-TS Batch Mode no Cell Type K5 Wavelength 6.560000000e+002 Calibration Constant 5.680000000e-005 Comet Cell Cleaner no Divide raw LS signal by laser monitor yes Divide raw LS signal by forward laser monitor no QELS Fiber Replaces Detector 0 Polarization Option no Temperature Controlled Line no Heated Line Temperature 0.000000000e+000 QELS Enabled no 1 44.7 0.798 2 90.0 1.000 3 135.3 0.811 Optilab rEX Temperature Control yes Temperature 2.500000000e+001 Band Broadening Correction n/a Disable Data Collection no Physical Instrument Name Optilab rEX Physical Instrument Location WYATT-869-rEX Batch Mode no Wavelength 6.580000000e+002 Calibration Constant 3.460689913e-005 Generic Pump Injector 0.00000000 Injector miniDAWN TREOS 0.00000000 miniDAWN TREOS Optilab rEX 0.14180000 Injector miniDAWN TREOS Collection Time Tuesday January 29, 2013 11:31:22 AM Eastern Daylight Time Processing time Tuesday January 29, 2013 03:47:10.281 PM Eastern Daylight Time Despiking Level Normal Basic Collection Collection Script File Name Inject to Collect Delay 0.000000000e+000 Collection Duration 4.000000000e+001 LS Instrument Collection Interval 5.000000000e-001 QELS Instrument Collection Interval 2.000000000e+000 Wait for Auto-inject Signal yes Define Baselines detector 1 7.39009735 0.08173509 29.84102600 0.08154900 manual x, auto y detector 2 9.77550852 0.03593920 25.72502242 0.03593872 manual x, auto y detector 3 8.09168887 0.06258811 26.80079608 0.06251280 manual x, auto y differential refractive index data 7.66286202 0.00000012 31.27075273 0.00000012 manual x, auto y Band Broadening Reference Instrument Optilab rEX Broaden Band Broadening Algorithm ASTRA V Define Peaks Peak Name Peak 1 Peak 2 Start 1.684449311e+001 1.637372292e+001 End 1.868049682e+001 1.679741609e+001 Light Scattering Model Zimm Zimm Fit Degree 1 1 dn/dc 1.850000000e-001 1.850000000e-001 dn/dc Ref. Temp. 2.500000000e+001 2.500000000e+001 Modifier dn/dc 0.000000000e+000 0.000000000e+000 Modifier dn/dc Ref. Temp. 2.500000000e+001 2.500000000e+001 A2 0.000000000e+000 0.000000000e+000 UV Ext. Coef. 0.000000000e+000 0.000000000e+000 UV Ext. Coef. Ref. Temp. 2.500000000e+001 2.500000000e+001 Modifier UV Ext. Coef. 0.000000000e+000 0.000000000e+000 Modifier UV Ext. Coef. Ref. Temp. 2.500000000e+001 2.500000000e+001 Real Refractive Index 0 0 Imaginary Refractive Index 0 0 Shell Thickness 0.000000000e+000 0.000000000e+000 Shell Real Refractive Index 0 0 Shell Imaginary Refractive Index 0 0 Rod radius 0.000000000e+000 0.000000000e+000 Molecular Standard n/a n/a Concentration 4.100000000e+000 4.100000000e+000 Concentration Ref. Temp. 2.500000000e+001 2.500000000e+001 Injected Mass 4.100000000e-003 0.000000000e+000 Molar Mass 0.000000000e+000 0.000000000e+000 Intrinsic Viscosity 0.000000000e+000 0.000000000e+000 Intrinsic Viscosity Ref. Temp. 2.500000000e+001 2.500000000e+001 Mark-Houwink-Sakurada K 0.000000000e+000 0.000000000e+000 Mark-Houwink-Sakurada a 0 0 Flory-Fox Equation Phi Parameter 0 0 Ptitsyn-Eizner Equation Epsilon Parameter 0 0 Viscometry Model Huggins Huggins Huggins Equation Parameter 0 0 Kraemers Equation Parameter 0 0 Radius 3.000000000e+000 3.000000000e+000 Radius Type rms rms Normalization Normalization Peak 1 Percent to Keep 0.000000000e+000 Molar Mass & Radius from LS Enabled Peaks 1 2 Enabled Detectors 1 2 3 Results Fitting Procedure laser monitor average 9.987998885e-001 Forward Monitor Average 7.461160376e-001 Calculated Mass 4.093570914e+002 3.876662431e+000 Mass Recovery 9.984319303e+006 n/a Mass Fraction 9.906187179e+001 9.381282132e-001 Peak Area 7.573106191e-005 7.171825498e-007 Peak Height 9.895898708e-005 2.283666350e-006 Retention Time 1.749923333e+001 1.671811667e+001 Peak Area % 9.906187179e+001 9.381282132e-001 Mn 1.958408651e+005 4.551462598e+005 Mp 2.003358105e+005 4.387024440e+005 Mv n/a n/a Mw 1.994318966e+005 4.568492152e+005 Mz 2.024120287e+005 4.585281082e+005 Mz+1 2.052916870e+005 4.601775991e+005 Mw/Mn 1.018336477e+000 1.003741556e+000 Mz/Mn 1.033553588e+000 1.007430245e+000 M(avg) 2.469766333e+005 4.898586854e+005 Rn 2.800937651e+001 3.398332352e+001 Std Dev Rn 1.528961278e+001 5.307249983e+000 Rw 2.757146415e+001 3.399645711e+001 Std Dev Rw 1.380077594e+001 5.396514492e+000 Rz 2.727279935e+001 3.401016670e+001 R(avg) 2.555852071e+001 3.509994334e+001 Average concentration 2.232792555e-001 9.019536799e-003 RMS conformation plot slope 3.791396109e-001 RMS Conformation Plot y-intercept -6.337998913e-001 Peak Area (detector 1) 1.905043816e-005 4.054532282e-007 1.879800372e-001 4.000806297e-003 Peak Area (detector 2) 1.774537362e-005 3.673982475e-007 1.397316545e-001 2.892988680e-003 Peak Area (detector 3) 1.722661769e-005 3.450156778e-007 1.672587379e-001 3.349867503e-003 Peak Height (detector 1) 2.510365422e-005 1.253534503e-006 2.477100955e-001 1.236924109e-002 Peak Height (detector 2) 2.357590907e-005 1.133962666e-006 1.856427964e-001 8.929114869e-003 Peak Height (detector 3) 2.287410480e-005 1.071669367e-006 2.220919954e-001 1.040518045e-002 Retention Time (detector 1) 1.747699967e+001 1.668259059e+001 Retention Time (detector 2) 1.748518945e+001 1.668259059e+001 Retention Time (detector 3) 1.747699967e+001 1.669078037e+001 Peak Area % (detector 1) 9.791603880e+001 2.083961203e+000 Peak Area % (detector 2) 9.797160672e+001 2.028393281e+000 Peak Area % (detector 3) 9.803651903e+001 1.963480972e+000